Composite

Part:BBa_K1339021:Design

Designed by: Madoka NAGATA   Group: iGEM14_Tokyo-NoKoGen   (2014-10-02)


Pbad-RBS-G3DH-T0 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3584
    Illegal NotI site found at 1565
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3890
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1708
    Illegal NgoMIV site found at 1805
    Illegal NgoMIV site found at 3021
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3870
    Illegal NgoMIV site found at 3935
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1987
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3078
    Illegal SapI site found at 961
    Illegal SapI site found at 3182


Design Notes

Pbad-RBS-G3DH-dT


Source

Pbad-RBS-G3DH-dT

References